Journal: The Journal of clinical endocrinology and metabolism

Article Title: Cyclooxygenase-2 Expression and Prostaglandin E 2 Synthesis Are Up-Regulated in Carcinomas of the Cervix: A Possible Autocrine/Paracrine Regulation of Neoplastic Cell Function via EP2/EP4 Receptors

doi:

Figure Lengend Snippet: A, Relative expression of COX-2 RNA in cervical squamous cell carcinoma (C1-C8), adenocarcinoma (C9 and C10), and normal cervix (N1-N5), as determined by real-time quantitative RT-PCR. B, Western blot analysis of 100 μg total protein isolated from human cervical carcinoma tissue. The proteins were loaded onto a 10% SDS-gel, electrophoresed, and subsequently transferred to a polyvinylidene difluoride membrane. The immunoblot was probed with antibody raised against the C-terminus of human COX-2. A specific band of approximately 72 kDa was detected in all squamous (C1-C8) and adenocarcinoma (C9 and C10). No signal was detected in normal cervical tissue (a representative sample is shown). Moreover, preadsorbing the antibody with the blocking peptide (B) abolished the COX-2 signal in all carcinoma samples (a representative sample is shown).

Article Snippet: Thereafter, membranes were incubated with goat anti-COX-2 primary IgG antibody (sc-1745, Autogenbioclear, Wiltshire, UK) at a dilution of 1:500 at4Cfor 18 h. Control samples were incubated with goat anti-COX-2 antibody preadsorbed to blocking peptide (sc-1745p, Autogenbioclear) according to the manufacturer’s protocol.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Isolation, SDS-Gel, Blocking Assay

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Cyclooxygenase-2 Expression and Prostaglandin E 2 Synthesis Are Up-Regulated in Carcinomas of the Cervix: A Possible Autocrine/Paracrine Regulation of Neoplastic Cell Function via EP2/EP4 Receptors

doi:

Figure Lengend Snippet: COX-2 expression and PGE2 synthesis are detected in epithelial cells of squamous cell carcinoma (A and B, respectively) and columnar and glandular epithelium of adenocarcinomas (C and D, respectively). Minimal COX-2 and PGE2 signal was detected in normal cervical tissue (E and F, respectively). Insets are serial sections that were stained with preadsorbed COX-2 or PGE2 serum, respectively (negative controls). Scale bar, 100 μm.

Article Snippet: Thereafter, membranes were incubated with goat anti-COX-2 primary IgG antibody (sc-1745, Autogenbioclear, Wiltshire, UK) at a dilution of 1:500 at4Cfor 18 h. Control samples were incubated with goat anti-COX-2 antibody preadsorbed to blocking peptide (sc-1745p, Autogenbioclear) according to the manufacturer’s protocol.

Techniques: Expressing, Staining

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Cyclooxygenase-2 Expression and Prostaglandin E 2 Synthesis Are Up-Regulated in Carcinomas of the Cervix: A Possible Autocrine/Paracrine Regulation of Neoplastic Cell Function via EP2/EP4 Receptors

doi:

Figure Lengend Snippet: COX-2 (A) expression and PGE2 (B) synthesis are detected in endothelial cells (arrowed) of all carcinoma tissues. Vascular endothelial cells in cervical cancer tissues were localized using antibodies raised against the human CD34 endothelial cell marker (C). D, A representative section incubated with nonimmune goat serum (CD34-negative control). Scale bar, 50 μm.

Article Snippet: Thereafter, membranes were incubated with goat anti-COX-2 primary IgG antibody (sc-1745, Autogenbioclear, Wiltshire, UK) at a dilution of 1:500 at4Cfor 18 h. Control samples were incubated with goat anti-COX-2 antibody preadsorbed to blocking peptide (sc-1745p, Autogenbioclear) according to the manufacturer’s protocol.

Techniques: Expressing, Marker, Incubation, Negative Control